ABSTRACT
Introduction: Clindamycin has been used to treat pneumoniaand soft tissue and musculoskeletal infections due to MRSA.One important problem in Clindamycin treatment is the riskof clinical failure during therapy caused by MLSB inducibleresistance. The Clinical and Laboratory Standards Institute(CLSI) suggest D-test, which is a phenotypic showingtechnique for inducible Clindamycin resistance.Material and Methods: We analyzed antimicrobialsusceptibility testing by Kirby Bauer disk diffusion method.Methicillin resistance was detected with cefoxitin (30 µg) diskand inducible clindamycin resistance was unwavering in allerythromycin resistant isolate by using D-zone test.Results: 100 S. aureus isolate 37 (36.6%) were methicillinresistant (MRSA) and 63 (63.4%) were methicillin-sensitiveS. aureus (MSSA). Although, mainstream of the MRSAisolates were imitative from pus samples 15, however, the S.aureus isolates imitative from post-operative wound infectionwere mainly MRSA 7. A total of 21 S. aureus isolates withiMLSB phenotype shown that they were 100% susceptible tovancomycin and linezolid, with modest sensitivity (71.14%) togentamicin, cefuroxime and slightest sensitivity to (23.81%)doxycycline, (20.95%) ciprofloxacin.Conclusion: Outstanding to high happening of erythromycinresistance amongst S. aureus isolates, we recommend thatD-zone test have to be regularly done in all laboratories forsuitable recommendation of clindamycin and thus preventingappearance of inducible resistant strains and managementfailure.
ABSTRACT
Introduction: Nosocomial infection is a major problem globally. Methicillin-resistant Staphylococcus aureus (MRSA) remains one of the most important causes of nosocomial infections worldwide. MRSA are the important agents causing nosocomial infections. The study was conducted in the Department of Microbiology to determine the prevalence of MRSA and antibiotic susceptibility pattern. Material and Methods: This was a retrospective study conducted from July 2016 to August 2017 in a tertiary care hospital in Northern Bihar India. All isolates were identified in patients and data provided by the Clinical and Laboratory Standards Institute (CLSI) guidelines and antibiotic susceptibility pattern considered by Kirby Bauer disc diffusion method. The information was definitely recorded and analyzed using Microsoft Excel 2007 edition. Results: 200 Staphylococcus strains isolate of were isolated from various clinical samples, Out of 200 S. aureus isolates, 73 (36.5%) were methicillin resistant S. aureus (MRSA) and 127 (63.5%) were methicillin-sensitive S. aureus (MSSA) in our labs. Although, the majority of the MRSA isolates were resulted from pus samples 30, however, the S. aureus isolates resulted from post-operative wound infection was mostly MRSA. Conclusion: In conclusion, the importance of Isolation of MRSA patients and carriers in the hospitals, regular surveillance of hospital associated infections including monitoring antibiotic sensitivity pattern and strict drug policy for antibiotics may be helpful for reducing the incidence of these infections